Mechanisms of action of cytoplasmic microRNAs. Part 1. Mechanisms of interaction of microRNA and mRNA molecules. Influence of microRNAs on translation




microRNA, miRNA, miR, mRNA translation, Argonaute proteins, review


The scientific review presents the mechanisms of action of cytoplasmic miRNAs, namely the relationship between miRNA and mRNA molecules and the influence of miRNAs on translation. To write the article, information was searched using Scopus, Web of Science, MEDLINE, PubMed, Google Scholar, Embase, Global Health, The Cochrane Library, CyberLeninka databases. The authors state that the interaction of microRNA and mRNA requires the presence in the region of the 3'-end of the mRNA molecule of small nucleotide sequences — miRNA regulatory elements, which are complementary to the sequences of the “seed” region of microRNA. It is known that only six nucleotide matches in the “seed” region (position 2–8) are required to initiate the interaction of microRNA with the mRNA target. It is emphasized that the interaction of miRNA with mRNA depends on the availability of the mRNA binding site. The authors suggest that accessory proteins are involved in the interaction of microRNA and mRNA. It is known that the process of mRNA and miRNA hybridization depends on the presence of SNP. Scientists believe that the main function of cytoplasmic miRNAs is to regulate the activity of protein synthesis. It is presented that microRNAs can repress and activate the mRNA translation process. In addition, some miRNAs are able to both inhibit and enhance the translation of mRNA depending on specific local conditions and the spectrum of microenvironmental factors. Thus, the mechanism of action of cytoplasmic miRNAs is realized due to the interaction of miRNAs and mRNAs, which is due to the presence of complementary nucleotide sequences of special regions. The interaction of miRNAs with mRNAs depends on the availability of the mRNA binding site, the involvement of accessory proteins, and the presence of SNP. Violations of microRNA-mRNA interactions lead to the development of pathological processes. Cytoplasmic miRNAs perform their main function, namely the regulation of protein synthesis activity, due to miRNA-mediated repression and activation of mRNA translation.


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Auton A, Brooks LD, Durbin RM et al. A global reference for human genetic variation. Nature. 2015 Oct 1;526(7571):68-74. doi:10.1038/nature15393.

Azlan A, Dzaki N, Azzam G. Argonaute: The executor of small RNA function. J Genet Genomics. 2016 Aug 20;43(8):481-494. doi:10.1016/j.jgg.2016.06.002.

Béthune J, Artus-Revel CG, Filipowicz W. Kinetic analysis reveals successive steps leading to miRNA-mediated silencing in mammalian cells. EMBO Rep. 2012 Aug;13(8):716-723. doi:10.1038/embor.2012.82.

Catalanotto C, Cogoni C, Zardo G. MicroRNA in Control of Gene Expression: An Overview of Nuclear Functions. Int J Mol Sci. 2016 Oct 13;17(10):1712. doi:10.3390/ijms17101712.

Detassis S, Grasso M, Del Vescovo V, Denti MA. MicroRNAs Make the Call in Cancer Personalized Medicine. Front Cell Dev Biol. 2017 Sep 22;5:86. doi:10.3389/fcell.2017.00086.

Djuranovic S, Nahvi A, Green R. MiRNA-mediated gene silencing by translational repression followed by mRNA deadenylation and decay. Science. 2012 Apr 13;336(6078):237-240. doi:10.1126/science.1215691.

Fabian MR, Sonenberg N, Filipowicz W. Regulation of mRNA translation and stability by microRNAs. Annu Rev Biochem. 2010;79:351-379. doi:10.1146/annurev-biochem-060308-103103.

Gebremedhn S, Ali A, Hossain M, et al. MicroRNA-Mediated Gene Regulatory Mechanisms in Mammalian Female Reproductive Health. Int J Mol Sci. 2021 Jan 19;22(2):938. doi:10.3390/ijms22020938.

Iwakawa HO, Tomari Y. The Functions of MicroRNAs: mRNA Decay and Translational Repression. Trends Cell Biol. 2015 Nov;25(11):651-665. doi:10.1016/j.tcb.2015.07.011.

Kang H. MicroRNA-Mediated Health-Promoting Effects of Phytochemicals. Int J Mol Sci. 2019 May 23;20(10):2535. doi:10.3390/ijms20102535.

Karlsen TH, Lammert F, Thompson RJ. Genetics of liver disease: From pathophysiology to clinical practice. J Hepatol. 2015 Apr;62(1 Suppl):S6-S14. doi:10.1016/j.jhep.2015.02.025.

King VM, Borchert GM. MicroRNA Expression: Protein Participants in MicroRNA Regulation. Methods Mol Biol. 2017;1617:27-37. doi:10.1007/978-1-4939-7046-9_2.

Li LJ, Leng RX, Fan YG, Pan HF, Ye DQ. Translation of noncoding RNAs: Focus on lncRNAs, pri-miRNAs, and circRNAs. Exp Cell Res. 2017 Dec 1;361(1):1-8. doi:10.1016/j.yexcr.2017.10.010.

Ma X, Tao R, Li L, et al. Identification of a 5‑microRNA signature and hub miRNA‑mRNA interactions associated with pancreatic cancer. Oncol Rep. 2019 Jan;41(1):292-300. doi:10.3892/or.2018.6820.

Manivannan A, Kim JH, Yang EY, et al. Next-Generation Sequencing Approaches in Genome-Wide Discovery of Single Nucleotide Polymorphism Markers Associated with Pungency and Disease Resistance in Pepper. Biomed Res Int. 2018 Jan 9;2018:5646213. doi:10.1155/2018/5646213.

Meijer HA, Kong YW, Lu WT, et al. Translational repression and eIF4A2 activity are critical for microRNA-mediated gene regulation. Science. 2013 Apr 5;340(6128):82-85. doi:10.1126/science.1231197.

Morozova N, Zinovyev A, Nonne N, Pritchard LL, Gorban AN, Harel-Bellan A. Kinetic signatures of microRNA modes of action. RNA. 2012 Sep;18(9):1635-55. doi:10.1261/rna.032284.112.

Nakano M, Nakajima M. Current knowledge of microRNA-mediated regulation of drug metabolism in humans. Expert Opin Drug Metab Toxicol. 2018 May;14(5):493-504. doi:10.1080/17425255.2018.1472237.

Oliveto S, Mancino M, Manfrini N, Biffo S. Role of microRNAs in translation regulation and cancer. World J Biol Chem. 2017 Feb 26;8(1):45-56. doi:10.4331/wjbc.v8.i1.45.

Oulas A, Karathanasis N, Louloupi A, et al. Prediction of miRNA targets. Methods Mol Biol. 2015;1269:207-229. doi:10.1007/978-1-4939-2291-8_13.

Peterson SM, Thompson JA, Ufkin ML, Sathyanarayana P, Liaw L, Congdon CB. Common features of microRNA target prediction tools. Front Genet. 2014 Feb 18;5:23. doi:10.3389/fgene.2014.00023.

Salomon WE, Jolly SM, Moore MJ, Zamore PD, Serebrov V. Single-Molecule Imaging Reveals that Argonaute Reshapes the Binding Properties of Its Nucleic Acid Guides. Cell. 2016 Jul 14;166(2):517-520. doi:10.1016/j.cell.2016.06.048.

Seok H, Ham J, Jang ES, Chi SW. MicroRNA Target Recognition: Insights from Transcriptome-Wide Non-Canonical Interactions. Mol Cells. 2016 May 31;39(5):375-81. doi:10.14348/molcells.2016.0013.

Shyu AB, Wilkinson MF, van Hoof A. Messenger RNA regulation: to translate or to degrade. EMBO J. 2008 Feb 6;27(3):471-481. doi:10.1038/sj.emboj.7601977.

Thomson DW, Bracken CP, Goodall GJ. Experimental strategies for microRNA target identification. Nucleic Acids Res. 2011 Sep 1;39(16):6845-6853. doi:10.1093/nar/gkr330.

Valinezhad Orang A, Safaralizadeh R, Kazemzadeh-Bavili M. Mechanisms of miRNA-Mediated Gene Regulation from Common Downregulation to mRNA-Specific Upregulation. Int J Genomics. 2014;2014:970607. doi:10.1155/2014/970607.

Yu D, Chen S, Li D, Knox B, Guo L, Ning B. FREMSA: A Method That Provides Direct Evidence of the Interaction between microRNA and mRNA. Methods Mol Biol. 2020;2102:557-566. doi:10.1007/978-1-0716-0223-2_30.

Zhang T, Wu YC, Mullane P, et al. FUS Regulates Activity of MicroRNA-Mediated Gene Silencing. Mol Cell. 2018 Mar 1;69(5):787-801.e8. doi:10.1016/j.molcel.2018.02.001.

Zhao S, Liu MF. Mechanisms of microRNA-mediated gene regulation. Sci China C Life Sci. 2009 Dec;52(12):1111-1116. doi:10.1007/s11427-009-0152-y.



How to Cite

Abaturov, A., & Babуch V. (2022). Mechanisms of action of cytoplasmic microRNAs. Part 1. Mechanisms of interaction of microRNA and mRNA molecules. Influence of microRNAs on translation. CHILD`S HEALTH, 17(2), 114–119.



Theoretical Medicine

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