DOI: https://doi.org/10.22141/2224-0551.6.49.2013.84834

Genetic Polymorphism of Toll-Like Receptor 4 ASP299GLY Gene Polymorphism in Children with Helicobacter pylori Infection

O.Ye. Abaturov, O.M. Gerasymenko, O.A. Shlykova, I.P. Kaidashev

Abstract


The objective of the study was to determine the characteristics of H.pylori infection in children with Toll-like receptor 4 (TLR4) ASP299GLY gene polymorphism.
Methods. 31 children aged 9 to 17 years with chronic gastroduodenal pathology were observed. The control group included 95 healthy individuals.
Results. Among children with chronic gastroduodenal pathology, 16.13 % had heterozygous genotype Asp/Gly, which is higher than in the control group (P = 0.01). It was shown that in H.pylori positive children the frequency of the heterozygous genotype Asp/Gly was higher than in H. pylori negative patients (17.4 and 12.5 %, respectively, OR = 1.48; P = 0.75). Among H.pylori positive children heterozygous genotype Asp/Gly was detected more frequently in children infected with СagA-positive strains of H.pylori, compared to patients with СagA-negative status (P > 0.5). It is proved that in children with TLR4 ASP299GLY gene polymorphism a decrease in the expression of TLR4 in biopsy sample of the gastric mucosa and water-soluble sCD14 levels was observed, together with prominent inflammatory mucosal changes (Pu < 0.05).
Conclusion. Among children with chronic inflammatory gastroduodenal diseases, genotype Asp/Gly SNP Asp299Gly of the gene TLR4 is observed significantly higher than in healthy population. Presence of genotype Asp/Gly in children predisposes to contamination with H.pylori.


Keywords


Helicobacter pylori infection; TLR4 ASP299GLY gene polymorphism; children

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