The Single-Nucleotide Polymorphism rs510432 in ATG5 Gene and the Development of Atopic March in Children

O.V. Iemets


Objective: to determine the association between single-nucleotide polymorphism rs510432 in gene ATG5 and the development of atopic march in children, to find out the probability of clinical manifestation and the features of the clinical course of atopic diseases in individuals with different allelic variants of polymorphism. Methods. Genotyping assay of polymorphism rs510432 in gene ATG5 was performed in children with atopic diseases and apparently healthy children using real-time polymerase chain reaction. Results. The minor T allele of rs510432 in ATG5 gene was significantly more often found in patients with atopic diseases than in apparently healthy children (χ2 = 6.36; р < 0.05). The results of logistic regression demonstrated that the risk of atopic diseases in children with minor genotype is 2.4 times higher than in carriers of major genotype. The average values of forced expiratory volume in 1 second (FEV1) are significantly lower in asthma patients with minor genotype than in thereof with major genotype (Q = 2.71; р < 0.05). There were no statistically significant differences in the distribution of allelic variants of rs510432 polymorphism of the gene ATG5 depending on the severity of bronchial asthma, atopic dermatitis and allergic rhinitis in children. Conclusions. Children with minor TT genotype of gene ATG5 polymorphism had an increased risk of atopic diseases, which is 72 %. In patients with bronchial asthma, minor TT genotype of gene ATG5 polymorphism has been associated with reduced FEV1. Rs510432 polymorphism of the gene ATG5 should be used to predict the development of atopic diseases in children.


single nucleotide polymorphism; autophagy; ATG5; atopic diseases; children


Lasitsya OL, Lasitsya TS, Nedelska SM authors, Radchenko NM editor. AlergologIya dityachogo viku [Allergy childhood]. Kyiv: Kniga plyus; 2004.368 p. Ukrainian

Ohotnikova EN [Mechanisms of formation and clinical features of the course "atopic march" in children] Zdorove Ukrainyi. 2010: 16-17. Russian.

Reken M, Grevers G, Burgdorf V authors, Reken M editor. Naglyadnaya allergologiya [Transparent allergology]. Moscow: Binom. Laboratoriya znaniy; 2013. 238 p. Russian.

Beck LA, Leung DY [Allergen sensitization through the skin induces systemic allergic responses] J Allergy Clin Immunol. 2000, 106(5 Suppl): 258-63. English.

Betz C, Hall MN [Where is mTOR and what is it doing there?] J Cell Biol. 2010, 203(4): P. 563–574. English.

Cookson WO, Ubhi B, Lawrence R, Abecasis GR, Walley AJ, Cox HE, Coleman R, Leaves NI, Trembath RC, Moffatt MF, Harper JI [Genetic linkage of childhood atopic dermatitis to psoriasis susceptibility loci] Nat Genet. 2001, 27(4): 372-3. English.

Deretic V [Autophagy in infection] Curr Opin Cell Biol. 2010, 22(2): 252–562. English.

Eichenfield LF, Hanifin JM, Beck LA, Lemanske RF Jr, Sampson HA, Weiss ST, Leung DY [Atopic dermatitis and asthma: parallels in the evolution of treatment] Pediatrics. 2003, 111(3): 608-16. English.

Gupta J, Johansson E, Bernstein JA, Chakraborty R, Khurana Hershey GK, Rothenberg ME, Mersha TB [Resolving the etiology of atopic disorders by using genetic analysis of racial ancestry] J Allergy Clin Immunol. 2016, pii: S0091-6749(16)30256-1. doi: 10.1016/j.jaci.2016.02.045. [Epub ahead of print]

Hussey S, Travassos LH, Jones NL [Autophagy as an emerging dimension to adaptive and innate immunity] Semin Immunol. 2009, 21: 233–41. English.

Inoue D, Kubo H, Taguchi K, Suzuki T, Komatsu M [Inducible disruption of autophagy in the lung causes airway hyper-responsiveness] Biochem Biophys Res Commun. 2011, 400: 13–18. English.

Jyothula SK, Eissa NT [Autophagy and role in asthma] Curr Opin Pulm Med. 2013, 19: 30–35. English.

Kondo N, Matsui E, Nishimura A, Kaneko H [Pharmacogenetics of asthma in children] Allergy Asthma Immunol Res. 2010, 2 (1): 14–9. English.

Lee JU, Kim JD, Park CS [Gene-Environment Interactions in Asthma: Genetic and Epigenetic Effects] Yonsei Med J. 2015, 56(4): 877–86. English.

Levine B, Deretic V [Unveiling the roles of autophagy in innate and adaptive immunity] Nat Rev Immunol. 2007, 7(10): 767–77. English.

Li G, Tang D, Lotze MT [Ménage à Trois in stress: DAMPs, redox and autophagy] Semin Cancer Biol. 2013, 23(5): 380–90. English.

Li C, Capan E, Zhao Y, Zhao J, Stolz D, Watkins SC, Jin S, Lu B [Autophagy is induced in CD4+ T cells and important for the growth factor-withdrawal cell death] J. Immunol. 2006, 177: 5163–5168. English.

Miller BC, Zhao Z, Stephenson LM, Cadwell K, Pua HH, Lee HK, Mizushima N N, Iwasaki A, He YW, Swat W, Virgin HW [The autophagy gene ATG5 plays an essential role in B lymphocyte development] Autophagy. 2008, 4(3): 309–14. English.

Mizushima N, Ohsumi Y, Yoshimori T [Autophagosome formation in mammalian cells] Cell Struct Funct. 2002, 27(6): 421–9. English.

Nakano H, Ushio H [An unexpected role for autophagy in degranulation of mast cells] Autophagy. 2011, 7(6): 657–9. English.

Oh JЕ, Lee HK [Autophagy as an innate immune modulator] Immune Network. 2013, 13(1): 1–9. English.

Ortiz RA, Barnes KC [Genetics of allergic diseases] Immunol Allergy Clin North Am. 2015, 35(1): 19–44. English.

Poon A, Eidelman D, Laprise C, Hamid Q [ATG5, autophagy and lung function in asthma] Autophagy. 2012, 8(4): 694–5. English.

Puleston DJ, Simon AK [Autophagy in the immune system] Immunology. 2014, 141(1): 1–8. English.

Schmid D, Pypaert M, Munz C [Antigen-loading compartments for major histocompatibility complex class II molecules continuously receive input from autophagosomes] Immunity. 2007, 26: 79–92. English.

Spergel JM, Paller AS [Atopic dermatitis and the atopic march] J Allergy Clin Immunol. 2003, 112 (6 Suppl.): 118-27. English.

Tao Zheng, Jinho Yu, Min Hee Oh, Zhou Zhu [The Atopic March: Progression from Atopic Dermatitis to Allergic Rhinitis and Asthma] Asthma Immunol Res. 2011, 3(2): 67–73. English.

Valdor R, Macian F [Autophagy and the regulation of the immune response] Pharmacological Research. 2012, 66: 475–483. English.

Xu Yi, Eissa NT [Autophagy in Innate and Adaptive Immunity] Proc Am Thorac Soc. 2010, 7: 22–28. English.

Zhifen Yang, Klionsky DJ [An Overview of the Molecular Mechanism of Autophagy] Curr Top Microbiol Immunol. 2009, 335: 1–32. English.

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