Frequency of Polymorphic Variants of Phase II Genes of GSTM1 and GSTT1 Xenobiotic Biotransformation in Newborns from Ivano-Frankivsk Region of Ukraine

N.G. Gorovenko, S.V. Podolska, Z.R. Kocherga

Abstract


To determine the allelic polymorphism of glutathione-S-transferases genes GSTM1 and GSTT1 in healthy newborns, premature infants and infants with intrauterine growth retardation we conducted molecular genetic study of umbilical cord blood. To determine the individual response of the genome of a newborn on the possible toxic effects we used the micronucleus test. It is found that in all newborns from the region the frequency of polymorphic variant GSTM1+ was 43.70 %, the frequency of GSTM1– polymorphic variant — 56.30 %. For GSTT1 gene frequency of polymorphic variant GSTT1+ and GSTT1– was 80.67 and 19.33 %, respectively. Positive micronucleus test in neonates was associated with polymorphic variants of GSTM1– — 71.95 % of cases compared with 21.62 % for negative micronucleus test. In the carriers of the GSTT1– polymorphic variant in any case there was no evidence of a positive micronucleus test, in all newborns with negative micronucleus test we found allelic variant GSTT1+. Newborns with negative micronucleus test are characterized by predominance (78.38 %) of genotypes GSTM1+/GSTT1+ association.


Keywords


allelic polymorphism; detoxication of xenobiotics; micronucleus test; newborns

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DOI: https://doi.org/10.22141/2224-0551.5.56.2014.76271

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