Allelic Polymorphism of enos Gene in Children Who Suffer from Bronchial Asthma, with Eosinophilic and Paucigranulocytic Subtypes of Bronchial Inflammation

L.O. Bezrukov, O.K. Koloskova, T.M. Bilous, N.K. Bohutska

Abstract


The objective of the research was to study the effect of allelic polymorphism of eNOS gene, encoding the activity of endothelial NO-synthase and synthesis of nitrogen monoxide in the airways, on some clinical and paraclinical parameters of bronchial asthma in school-age children with eosinophilic and paucigranulocytic inflammatory subtypes.
We have examined 22 patients with bronchial asthma with eosinophilic nature of the airway inflammation and 15 patients — with paucigranulocytic one. The immunological, spirometric studies, genotyping of endothelial NOS were carried out.
The severity of bronchial asthma in patients with different types of bronchial inflammation doesn’t depend on allelic polymorphism of the eNOS gene. The absence ofeNOS gene mutations as GG genotype was characteristic of patients with paucigranulocytic bronchial inflammation, allelic polymorphism GT/TT — of children with eosinophilic inflammation. In the presence of allelic polymorphism of eNOS gene as 
GT/TT genotype, children with paucigranulocytic bronchial inflammation were characterized by more frequent clinical manifestations of atopic dermatitis and allergic rhinitis, higher bronchial lability index and lower concentrations of NO metabolites in the exhaled breath condensate.


Keywords


bronchial asthma; children; type of inflammation; eNOS genotype.

References


Pedersen S.E., Hurd S.S., Lemanske R.F. Global strategy for the diagnosis and management of asthma in children 5 years and younger. Pediatr. Pulmonol. 2011;46:1-17. doi: 10.1002/ppul.21321.

Bacharier L.B., Boner A., Carlsen K.H. Diagnosis and treatment of asthma in childhood: aPRACTALL consensus report. Allergy. 2008; 63: 5-34.

Babadzhan N.D. Imuno-patohenetychni diahnostychni aspekty zapal'nykh fenotypiv bronkhial'noyi astmy / N.D. Babadzhan, L.V.Kuznetsova, H.I.Nazarenko, I.A.Asyka // Imunolohiya ta alerholohiya: nauka і praktyka.- 2011.- #3.- S.51-60.

Simpson J.L., Scott R., Boyle M.J., Gibson P.G. Inflammatory subtypes in asthma: assessment and identification using induced sputum. Respirology. 2006; 11:54-61. doi: 10.1111/j. 1440-1843.2006.00784.x

Broeke R.T., De Crom R., Van Haperen R. Over expression of endothelial nitric oxide synthase suppresses features of allergic in mice. Respir. Res.2006;7:7-58. doi: 10.1186/1465-9921-7-58

Yanamandra K., Boggs P.B., Thurmon T.F. Novel allele of endothelial nitric oxide synthase gene polymorphism in Caucasian asthmatics. Biochem. Biophys. Res. Commun. 2005; 335:545-549. doi: 10.1016/j.bbrc.2005.07.108.

Meurs H., Gosens R., Zaagsma J. Airway hyperresponsiveness in asthma: lessons from in vitro model systems and animal models. Ear. Respir. J. 2008;32:487-502. doi: 10.1183/09031936.00023608.

Kabayashi K., Nishimura Y., Yamashita T. The effect of overexpression of endothelial nitric oxide synthase on eosinophilic lung inflammation in a murine model. Int. Immunopharmacol. 2006;6:1040-1052. doi: 10.1016/j.intimp.2005.09.016

Kurbacheva O.M. Sovremennyy podkhod k vyboru terapiyi bronkhyal'noy astmy: ot ponimaniya klinicheskikh fenotipov k prakticheskim aspektam / О М.Kurbacheva, K.S.Pavlova, Y.E.Kozulyna // RMZh. Bolezni dykhatel'nykh putey. - 2013.- T.21 - #.29.- S. 1452-1460.

Pertseva T.A. Diahnosticheskaya traktovka ponyatiya «bronkhial'naya astma». Fenotipy і uroven' kontrolya nad zabolevaniyem [Elektronnyy resurs] / T. A. Pertseva. - Rezhym dostupa: http://www.likar.info/biblioteka/50056/

Emchenko N.L. Unyversal'nyy metod opredeleniya nitratov v biosredakh orhanizma / N.L.Emchenko, O.Y.Tsyhanenko. T.V.Koval'skaya // Klinicheskaya і laboratornaya diahnostika.- 1994. - #6.- S. 19-20.

Pavord I.D., Pizzichini M.M.. Pizzichini E. The use of induced sputum to investigate airway inflammation. Thorax. 1997:52:498-501. doi: 10.1136/thx.52.6.498

Lex C, Rayne D.N.R.. Zacharasiewich A. Sputum induction in children with difficult asthma: safety, feasibility and inflammatory cell patterns. Pediatr. Pulmon. 2005;39:318-324. doi: 10.1002/ppul.20159

Vydeleniye DNK iz krovi / Prakticheskaya molekulyarnaya biolohiya [Elektronnyy resurs]. Rezhym dostupa: http://molbiol.edu.ru/map.html

Ricciardolo F.L. Multiple roles of nitric oxide in the airways. Thorax. 2003;58:175-182. doi: 10.1136/thorax.58.2. 175




DOI: https://doi.org/10.22141/2224-0551.3.63.2015.75181

Refbacks

  • There are currently no refbacks.


Copyright (c) 2016 CHILD`S HEALTH

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

 

© Publishing House Zaslavsky, 1997-2017

 

 Яндекс.МетрикаSeo анализ сайта Рейтинг@Mail.ru