Bacterial surfactants as agents with antibiofilm activity
Keywords:bacterial biofilms, dispersion, biosurfagents
Biosuragents are a heterogeneous group of biological surface-active amphiphilic compounds. The producers of biosurfactants are various microorganisms: bacteria and fungi. The class of biosurfactants consists of two groups: low molecular weight and high molecular weight compounds. Representatives of low molecular weight compounds are lipopeptides, glycolipids, fatty acids, phospholipids that reduce surface and interfacial tension, and high molecular weight compounds are polymer and dispersed biosurfactants, which are emulsion stabilizers. The most studied biosurfactants with the potential of drugs are lipopeptides and glycolipids. A subgroup of lipopeptides are polymyxins, pseudo-factins, putisolvins, surfactin, fengycin and others; and glycolipids — rhamnolipids, trehalose, sophorose, cellobiose, mannosileritritol lipids, and others. Biosurfactants play a key role in the life of biofilms: they regulate the adhesion of bacteria and biofilm matrix, support the functioning of the matrix channels, providing the nutrient needs of bacteria. It has also been shown that biosurfactants are involved in the formation and dispersion of formed biofilms. These substances, directly reacting with the components of the matrix, induce degradation of the biofilm. Biosurfing agents, possessing antimicrobial, antifungal and antiviral, and antitumor properties, are a promising class of compounds that, possessing a combination of antibacterial and antibiofilm action, open up new perspectives in the treatment of recurrent chronic infectious diseases. It is believed that surface-active compounds, both representatives of lipopeptides and glycolipids, can be the molecular basis for the development of drugs that will enhance the effectiveness of antibiotic therapy for problem infections, especially those caused by antibiotic-resistant strains.
Balleza D, Alessandrini A, Beltrán García MJ. Role of Lipid Composition, Physicochemical Interactions, and Membrane Mechanics in the Molecular Actions of Microbial Cyclic Lipopeptides. J Membr Biol. 2019 Jun;252(2-3):131-157. doi:10.1007/s00232-019-00067-4.
Banat IM, Franzetti A, Gandolfi I, Bestetti G, Martinotti MG, Fracchia L, Smyth TJ, Marchant R. Microbial biosurfactants production, applications and future potential. Appl Microbiol Biotechnol. 2010 Jun;87(2):427-44. doi:10.1007/s00253-010-2589-0.
Berditsch M, Jäger T, Strempel N, Schwartz T, Overhage J, Ulrich AS. Synergistic effect of membrane-active peptides polymyxin B and gramicidin S on multidrug-resistant strains and biofilms of Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2015 Sep;59(9):5288-96. doi:10.1128/AAC.00682-15.
Biniarz P, Coutte F, Gancel F, Łukaszewicz M. High-throughput optimization of medium components and culture conditions for the efficient production of a lipopeptide pseudofactin by Pseudomonas fluorescens BD5. Microb Cell Fact. 2018 Aug 4;17(1):121. doi:10.1186/s12934-018-0968-x.
Bionda N, Fleeman RM, de la Fuente-Núñez C, et al. Identification of novel cyclic lipopeptides from a positional scanning combinatorial library with enhanced antibacterial and antibiofilm activities. Eur J Med Chem. 2016 Jan 27;108:354-363. doi:10.1016/j.ejmech.2015.11.032.
Brasseur R, Braun N, El Kirat K, Deleu M, Mingeot-Leclercq MP, Dufrêne YF. The biologically important surfactin lipopeptide induces nanoripples in supported lipid bilayers. Langmuir. 2007 Sep 11;23(19):9769-72. doi:10.1021/la7014868.
Ceresa C, Tessarolo F, Maniglio D, et al. Medical-Grade Silicone Coated with Rhamnolipid R89 Is Effective against Staphylococcus spp. Biofilms. Molecules. 2019 Oct 25;24(21):3843. doi:10.3390/molecules24213843.
Díaz De Rienzo MA, Banat IM, Dolman B, Winterburn J, Martin PJ. Sophorolipid biosurfactants: Possible uses as antibacterial and antibiofilm agent. N Biotechnol. 2015 Dec 25;32(6):720-6. doi:10.1016/j.nbt.2015.02.009.
Diaz De Rienzo MA, Stevenson PS, Marchant R, Banat IM. Effect of biosurfactants on Pseudomonas aeruginosa and Staphylococcus aureus biofilms in a BioFlux channel. Appl Microbiol Biotechnol. 2016 Jul;100(13):5773-9. doi:10.1007/s00253-016-7310-5.
Fenibo EO, Ijoma GN, Selvarajan R, Chikere CB. Microbial Surfactants: The Next Generation Multifunctional Biomolecules for Applications in the Petroleum Industry and Its Associated Environmental Remediation. Microorganisms. 2019 Nov 19;7(11):581. doi:10.3390/microorganisms7110581.
Fernández-Barat L, Ciofu O, Kragh KN, et al. Phenotypic shift in Pseudomonas aeruginosa populations from cystic fibrosis lungs after 2-week antipseudomonal treatment. J Cyst Fibros. 2017 Mar;16(2):222-229. doi:10.1016/j.jcf.2016.08.005.
Hanif A, Zhang F, Li P, et al. Fengycin Produced by Bacillus amyloliquefaciens FZB42 Inhibits Fusarium graminearum Growth and Mycotoxins Biosynthesis. Toxins (Basel). 2019 May 24;11(5):295. doi:10.3390/toxins11050295.
Janek T, Łukaszewicz M, Krasowska A. Antiadhesive activity of the biosurfactant pseudofactin II secreted by the Arctic bacterium Pseudomonas fluorescens BD5. BMC Microbiol. 2012 Feb 23;12:24. doi:10.1186/1471-2180-12-24.
Janek T, Łukaszewicz M, Rezanka T, Krasowska A. Isolation and characterization of two new lipopeptide biosurfactants produced by Pseudomonas fluorescens BD5 isolated from water from the Arctic Archipelago of Svalbard. Bioresour Technol. 2010 Aug;101(15):6118-23. doi:10.1016/j.biortech.2010.02.109.
Klinger-Strobel M, Stein C, Forstner C, Makarewicz O, Pletz MW. Effects of colistin on biofilm matrices of Escherichia coli and Staphylococcus aureus. Int J Antimicrob Agents. 2017 Apr;49(4):472-479. doi:10.1016/j.ijantimicag.2017.01.005.
Kolpen M, Appeldorff CF, Brandt S, et al. Increased bactericidal activity of colistin on Pseudomonas aeruginosa biofilms in anaerobic conditions. Pathog Dis. 2016 Feb;74(1):ftv086. doi:10.1093/femspd/ftv086.
Kulakovskaya E, Kulakovskaya T. Chapter 1 - Structure and Occurrence of Yeast Extracellular Glycolipids. In: Kulakovskaya E, Kulakovskaya T, eds. Extracellular Glycolipids of Yeasts. Academic Press; 2014. pp 1-13. doi: 10.1016/B978-0-12-420069-2.00001-7.
Malinowski AM, McClarty BM, Robinson C, Spear W, Sanchez M, Sparkes TC, Brooke JS. Polysorbate 80 and polymyxin B inhibit Stenotrophomonas maltophilia biofilm. Diagn Microbiol Infect Dis. 2017 Feb;87(2):154-156. doi:10.1016/j.diagmicrobio.2016.11.008.
Ongena M, Jourdan E, Adam A, et al. Surfactin and fengycin lipopeptides of Bacillus subtilis as elicitors of induced systemic resistance in plants. Environ Microbiol. 2007 Apr;9(4):1084-90. doi:10.1111/j.1462-2920.2006.01202.x.
Poirel L, Jayol A, Nordmann P. Polymyxins: Antibacterial Activity, Susceptibility Testing, and Resistance Mechanisms Encoded by Plasmids or Chromosomes. Clin Microbiol Rev. 2017 Apr;30(2):557-596. doi:10.1128/CMR.00064-16.
Raaijmakers JM, De Bruijn I, Nybroe O, Ongena M. Natural functions of lipopeptides from Bacillus and Pseudomonas: more than surfactants and antibiotics. FEMS Microbiol Rev. 2010 Nov;34(6):1037-62. doi:10.1111/j.1574-6976.2010.00221.x.
Rivardo F, Turner RJ, Allegrone G, Ceri H, Martinotti MG. Anti-adhesion activity of two biosurfactants produced by Bacillus spp. prevents biofilm formation of human bacterial pathogens. Appl Microbiol Biotechnol. 2009 Jun;83(3):541-53. doi:10.1007/s00253-009-1987-7.
Roy A, Mahata D, Paul D, Korpole S, Franco OL, Mandal SM. Purification, biochemical characterization and self-assembled structure of a fengycin-like antifungal peptide from Bacillus thuringiensis strain SM1. Front Microbiol. 2013 Nov 21;4:332. doi:10.3389/fmicb.2013.00332.
Salman M, Rizwana R, Khan H, et al. Synergistic effect of silver nanoparticles and polymyxin B against biofilm produced by Pseudomonas aeruginosa isolates of pus samples in vitro. Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):2465-2472. doi:10.1080/21691401.2019.1626864.
Schneider-Futschik EK, Paulin OKA, Hoyer D, et al. Sputum Active Polymyxin Lipopeptides: Activity against Cystic Fibrosis Pseudomonas aeruginosa Isolates and Their Interactions with Sputum Biomolecules. ACS Infect Dis. 2018 May 11;4(5):646-655. doi:10.1021/acsinfecdis.7b00238.
Vanittanakom N, Loeffler W, Koch U, Jung G. Fengycin--a novel antifungal lipopeptide antibiotic produced by Bacillus subtilis F-29-3. J Antibiot (Tokyo). 1986 Jul;39(7):888-901. doi:10.7164/antibiotics.39.888.
Vollenbroich D, Ozel M, Vater J, Kamp RM, Pauli G. Mechanism of inactivation of enveloped viruses by the biosurfactant surfactin from Bacillus subtilis. Biologicals. 1997 Sep;25(3):289-97. doi:10.1006/biol.1997.0099.
Wang J, Yu B, Tian D, Ni M. Rhamnolipid but not motility is associated with the initiation of biofilm seeding dispersal of Pseudomonas aeruginosa strain PA17. J Biosci. 2013 Mar;38(1):149-56. doi:10.1007/s12038-012-9297-0.
Ye L, Hildebrand F, Dingemans J, et al. Draft genome sequence analysis of a Pseudomonas putida W15Oct28 strain with antagonistic activity to Gram-positive and Pseudomonas sp. pathogens. PLoS One. 2014 Nov 4;9(11):e110038. doi:10.1371/journal.pone.0110038.
Zheng H, Singh N, Shetye GS, Jin Y, Li D, Luk YY. Synthetic analogs of rhamnolipids modulate structured biofilms formed by rhamnolipid-nonproducing mutant of Pseudomonas aeruginosa. Bioorg Med Chem. 2017 Mar 15;25(6):1830-1838. doi:10.1016/j.bmc.2017.01.042.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Our edition uses the copyright terms of Creative Commons for open access journals.
Authors, who are published in this journal, agree with the following terms:
- The authors retain rights for authorship of their article and grant to the edition the right of first publication of the article on a Creative Commons Attribution 4.0 International License, which allows others to freely distribute the published article, with the obligatory reference to the authors of original works and original publication in this journal.
- Directing the article for the publication to the editorial board (publisher), the author agrees with transmitting of rights for the protection and using the article, including parts of the article, which are protected by the copyrights, such as the author’s photo, pictures, charts, tables, etc., including the reproduction in the media and the Internet; for distributing; for the translation of the manuscript in all languages; for export and import of the publications copies of the writers’ article to spread, bringing to the general information.
- The rights mentioned above authors transfer to the edition (publisher) for the unlimited period of validity and on the territory of all countries of the world.
- The authors guarantee that they have exclusive rights for using of the article, which they have sent to the edition (publisher). The edition (the publisher) is not responsible for the violation of given guarantees by the authors to the third parties.
- The authors have the right to conclude separate supplement agreements that relate to non-exclusive distribution of their article in the form in which it had been published in the journal (for example, to upload the work to the online storage of the journal or publish it as part of a monograph), provided that the reference to the first publication of the work in this journal is included.
- The policy of the journal permits and encourages the publication of the article in the Internet (in institutional repository or on a personal website) by the authors, because it contributes to productive scientific discussion and a positive effect on efficiency and dynamics of the citation of the article.
- The rights to the article are deemed transferred by the authors to the edition (the publisher) since the moment of the publication of the article in the printed or electronic version of journal.