Anti-biofilm activity of anti-matrix molecules
Keywords:bacterial biofilms, dispersion, anti-matrix molecules
The extracellular matrix of biofilms ensures the fixation of the biofilm on the biological surface and the protection of its bacteria from external adverse factors. The main structural component of biofilms is an extracellular polysaccharide substance. Exopolysaccharides and amyloid-like fibers are considered key molecular structures that support the three-dimensional structure of biofilms. Until recently, it was assumed that most biofilm dispersion mechanisms are associated with the functioning of matrix degrading enzymes, such as glycoside hydrolases, polysaccharide lyases, and proteases. However, it has been demonstrated that small molecules play an independent role in the process of destruction of matrix exopolysaccharides and amyloid-like fibers. Among the compounds that violate the biofilm matrix, anti-matrix molecules, compounds interacting with microdomains of the bacterial membrane and bacterial surfactants (biosurfactants) are distinguished. It has been demonstrated that compounds of these groups can inhibit the formation of biofilms and contribute to the dispersion of biofilms. From the group of anti-matrix molecules, the polyamine compound norspermidine interacts with exopolysaccharides, and the derivatives of benzoquinone AA-861 and sesquiterpene lactone parthenolide interact with TasA-amyloid-like fibers. Norspermidine prevents the formation and dispersion of biofilms of various bacteria, including Acinetobacter baumannii, Bacillus subtili, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis. Compound AA-861 is active against biofilms, which are formed by the bacteria Streptococcus mutans, Bacillus cereus, Escherichia coli. Parthenolide disperses biofilms formed by Escherichia coli and Bacillus cereus. Zaragozic acid, interacting with microdomains of the bacterial membrane, disruptі the functioning of raft-associated bacterial proteins. Small anti-matrix molecules and bacterial membranes aimed at microdomains that initiate biofilm dispersion will certainly become the basis for the development of effective antibiofilm therapeutic drugs.
Bergstrom JD, Kurtz MM, Rew DJ, et al. Zaragozic acids: a family of fungal metabolites that are picomolar competitive inhibitors of squalene synthase. Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):80-4. doi: 10.1073/pnas.90.1.80.
Besingi RN, Wenderska IB, Senadheera DB, et al. Functional amyloids in Streptococcus mutans, their use as targets of biofilm inhibition and initial characterization of SMU_63c. Microbiology. 2017 Apr;163(4):488-501. doi: 10.1099/mic.0.000443.
Cardile AP, Woodbury RL, Sanchez CJ Jr, et al. Activity of Norspermidine on Bacterial Biofilms of Multidrug-Resistant Clinical Isolates Associated with Persistent Extremity Wound Infections. Adv Exp Med Biol. 2017;973:53-70. doi: 10.1007/5584_2016_93.
Duanis-Assaf D, Duanis-Assaf T, Zeng G, et al. Cell wall associated protein TasA provides an initial binding component to extracellular polysaccharides in dual-species biofilm. Sci Rep. 2018 Jun 19;8(1):9350. doi: 10.1038/s41598-018-27548-1.
Hobley L, Kim SH, Maezato Y, Wyllie S, Fairlamb AH, Stanley-Wall NR, Michael AJ Norspermidine is not a self-produced trigger for biofilm disassembly. Cell. 2014 Feb 13;156(4):844-54. doi: 10.1016/j.cell.2014.01.012.
Kalia M, Yadav VK, Singh PK, Sharma D, Narvi SS, Agarwal V. Exploring the impact of parthenolide as anti-quorum sensing and anti-biofilm agent against Pseudomonas aeruginosa. Life Sci. 2018 Apr 15;199:96-103. doi: 10.1016/j.lfs.2018.03.013.
Kolodkin-Gal I, Cao S, Chai L, Böttcher T, Kolter R, Clardy J, Losick R. A self-produced trigger for biofilm disassembly that targets exopolysaccharide. Cell. 2012 Apr 27;149(3):684-92. doi: 10.1016/j.cell.2012.02.055.
Konai MM, Adhikary U, Samaddar S, Ghosh C, Haldar J. Structure-Activity Relationship of Amino Acid Tunable Lipidated Norspermidine Conjugates: Disrupting Biofilms with Potent Activity against Bacterial Persisters. Bioconjug Chem. 2015 Dec 16;26 (12):2442-53. doi: 10.1021/acs.bioconjchem.5b00494.
Li HY, Appelbaum FR, Willman CL, Zager RA, Banker DE. Cholesterol-modulating agents kill acute myeloid leukemia cells and sensitize them to therapeutics by blocking adaptive cholesterol responses. Blood. 2003 May 1;101(9):3628-34.
Liu CI, Jeng WY, Chang WJ, Ko TP, Wang AH. Binding modes of zaragozic acid A to human squalene synthase and staphylococcal dehydrosqualene synthase. J Biol Chem. 2012 May 25;287(22):18750-7. doi: 10.1074/jbc.M112.351254.
Mathema VB, Koh YS, Thakuri BC, Sillanpää M. Parthenolide, a sesquiterpene lactone, expresses multiple anti-cancer and anti-inflammatory activities. Inflammation. 2012 Apr;35(2):560-5. doi: 10.1007/s10753-011-9346-0.
Nesse LL, Berg K, Vestby LK. Effects of norspermidine and spermidine on biofilm formation by potentially pathogenic Escherichia coli and Salmonella enterica wild-type strains. Appl Environ Microbiol. 2015 Mar;81(6):2226-32. doi: 10.1128/AEM.03518-14.
Nishio T, Yoshikawa Y, Shew CY, Umezawa N, Higuchi T, Yoshikawa K. Specific effects of antitumor active norspermidine on the structure and function of DNA. Sci Rep. 2019 Oct 18;9(1):14971. doi: 10.1038/s41598-019-50943-1.
Qu L, She P, Wang Y, Liu F, Zhang D, Chen L, Luo Z, Xu H, Qi Y, Wu Y. Effects of norspermidine on Pseudomonas aeruginosa biofilm formation and eradication. Microbiologyopen. 2016 Jun;5(3):402-12. doi: 10.1002/mbo3.338.
Ramón-Peréz ML, Díaz-Cedillo F, Contreras-Rodríguez A, et al. Different sensitivity levels to norspermidine on biofilm formation in clinical and commensal Staphylococcus epidermidis strains. Microb Pathog. 2015 Feb;79:8-16. doi: 10.1016/j.micpath.2014.12.004.
Schwartz K, Syed AK, Stephenson RE, Rickard AH, Boles BR. Functional amyloids composed of phenol soluble modulins stabilize Staphylococcus aureus biofilms. PLoS Pathog. 2012;8(6):e1002744. doi: 10.1371/journal.ppat.1002744.
Copyright (c) 2020 А.Е. Abaturov
This work is licensed under a Creative Commons Attribution 4.0 International License.
Our edition uses the copyright terms of Creative Commons for open access journals.
Authors, who are published in this journal, agree with the following terms:
- The authors retain rights for authorship of their article and grant to the edition the right of first publication of the article on a Creative Commons Attribution 4.0 International License, which allows others to freely distribute the published article, with the obligatory reference to the authors of original works and original publication in this journal.
- Directing the article for the publication to the editorial board (publisher), the author agrees with transmitting of rights for the protection and using the article, including parts of the article, which are protected by the copyrights, such as the author’s photo, pictures, charts, tables, etc., including the reproduction in the media and the Internet; for distributing; for the translation of the manuscript in all languages; for export and import of the publications copies of the writers’ article to spread, bringing to the general information.
- The rights mentioned above authors transfer to the edition (publisher) for the unlimited period of validity and on the territory of all countries of the world.
- The authors guarantee that they have exclusive rights for using of the article, which they have sent to the edition (publisher). The edition (the publisher) is not responsible for the violation of given guarantees by the authors to the third parties.
- The authors have the right to conclude separate supplement agreements that relate to non-exclusive distribution of their article in the form in which it had been published in the journal (for example, to upload the work to the online storage of the journal or publish it as part of a monograph), provided that the reference to the first publication of the work in this journal is included.
- The policy of the journal permits and encourages the publication of the article in the Internet (in institutional repository or on a personal website) by the authors, because it contributes to productive scientific discussion and a positive effect on efficiency and dynamics of the citation of the article.
- The rights to the article are deemed transferred by the authors to the edition (the publisher) since the moment of the publication of the article in the printed or electronic version of journal.