Pathogenetic role of nitrosative and oxidative stress in the development of anemia of inflammation in young children
Background. The purpose was to study the pathogenetic role of nitrosative and oxidative stress in the occurrence of anemia of inflammation in young children. Materials and methods. The content of nitrotyrosine and phospholipase A2 in the blood serum of 55 young children (the average age of 1.6 ± 0.3 years) was determined by the method of enzyme-linked immunosorbent assay. The basic group consisted of 30 children with acute bacterial diseases of the respiratory tract: 21 patients were diagnosed with acute bacterial bronchitis, and 9 children — with community-acquired pneumonia. The basic group was divided into two subgroups: the first subgroup consisted of 15 children with anemia of inflammation, the second subgroup — 15 children with acute bacterial diseases of the respiratory tract without anemia manifestation. The comparison group included 10 children with iron deficiency anemia without manifestations of inflammatory diseases of the respiratory system. Fifteen apparently healthy children represented the control group. Results. It was found that anemia of inflammation in children is accompanied by the activation of nitrosative and oxidative stress as evidenced by high nitrotyrosine content (63.3 ± 4.7 ng/ml), which was 5 times greater than in the control group (12.5 ± 1.6 ng/ml) (p < 0.01) and phospholipase A2 level (6.1 ± 0.7 ng/ml), which was 2.3 times higher than in the control group (2.28 ± 0.40 ng/ml) (p < 0.05). The positive correlation was determined between the severity of bacterial inflammatory disease and the activation of nitrosative and oxidative stress (r = 0.7, p < 0.001). Conclusions. The activation of oxygen-containing and nitrogen-containing metabolites against the background of infectious and inflammatory disease induces the development of nitrosative and oxidative stress, which play an important role in the pathogenesis of anemia of inflammation in young children with acute bacterial respiratory diseases.
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