Blood levels of nitric oxide and endothelin-1 in Helicobacter pylori-associated peptic ulcer in children

Authors

  • T.V. Sorokman Department of pediatrics and medical genetics, Bukovinian State Medical University, Chernivtsi, Ukraine http://orcid.org/0000-0001-7615-3466
  • N.Ya. Cherney Department of pediatrics and medical genetics, Bukovinian State Medical University, Chernivtsi, Ukraine

DOI:

https://doi.org/10.22141/2224-0551.14.5.2019.177404

Keywords:

children, erosive-ulcerative pathology of the upper gastrointestinal tract, nitric oxide, endothelin-1

Abstract

Background. In recent years, many scientists emphasize the decisive role of the vascular wall in regulating the aggregate state of the blood, as well as the importance of its disorders in the deve­lopment of digestive diseases. However, there are currently no data on the role of Helicobacter pylori in the development of endothelial dysfunction. The purpose was to evaluate the nature of nitric oxide (NO) and endothelin-1 changes in the blood of children with erosive-ulcerative lesions of the upper gastrointestinal tract. Materials and methods. Comprehensive clinical and instrumental-laboratory examination was performed in 120 children aged 7–18 years, 79 with chronic erosive gastroduodenitis and 41 with duodenal ulcer, as study group, and 50 children of the corresponding age without digestive diseases (control group). All children underwent a tho­rough clinical and paraclinical study according to methods generally accepted in the clinic. Concentration of nitrite in the blood plasma was determined by P.P. Golikov (2004). The study of endothelin-1 serum level was carried out by enzyme-linked immunosorbent assay using Biomedica Gruppe (Germany) sets. The results of the study are represented by the number of observations in the group, the percentage or the mean and the mean square deviation, the exact value of p. Results. Children were divided by age, sex, place of residence and nosological form of pathology. The level of NO in the blood plasma of children in the study group was significantly lower — 11.78 ± 1.10 μmol/l (p < 0.05). The intragroup analysis in both groups of examined children showed no significant difference in the blood content of NO (p > 0.05) depending on gender and age. In the absence of Helicobacter pylori, the level of NO in the blood is significantly higher compared to the group of patients diagnosed with Helicobacter pylori (p < 0.05). There is a difference in the levels of NO in the blood of children depending on the period of the disease: they are higher during the exacerbation, both chronic erosive gastroduodenitis and duodenal ulcer. On average, blood endothelin-1 level in patients of the study group was significantly lower than that of children in the comparison group (0.36 ± 0.02 fmol/ml and 0.48 ± 0.02 fmol/ml, respectively, p < 0.01). The level of endothelin-1 in the blood of children also depended on the presence of Helicobacter pylori. Conclusions. The levels of NO and endothelin-1 metabolites are significantly reduced in the blood of children with erosive and ulcerative patho­logy of the upper gastrointestinal tract, depend on the period of the disease, the presence of Helicobacter pylori and do not depend on age and gender.

Author Biographies

T.V. Sorokman, Department of pediatrics and medical genetics, Bukovinian State Medical University, Chernivtsi, Ukraine

MD, PhD, Professor

N.Ya. Cherney, Department of pediatrics and medical genetics, Bukovinian State Medical University, Chernivtsi, Ukraine

Postgraduate student

References

Kotilea K, Bontems P, Touati E. Epidemiology, Diagnosis and Risk Factors of Helicobacter pylori Infection. Adv Exp Med Biol. 2019 Apr 24. doi: 10.1007/5584_2019_357.

Burgard M, Kotilea K, Mekhael J, et al. Evolution of Helicobacter pylori associated with gastroduodenal ulcers or erosions in children over the past 23 years: Decline or steady state? Helicobacter. 2019 Jul 8:e12629. doi: 10.1111/hel.12629.

Okuda M, Lin Y, Kikuchi S. Helicobacter pylori Infection in Children and Adolescents. Adv Exp Med Biol. 2019 Apr 30. doi: 10.1007/5584_2019_361.

Tsvetkova LN, Goriacheva OA, Tsvetkov PM, et al. Gastroenterological pathology in children: pathomorphism of diseases and improvement of diagnostic methods at the present stage. In: Mukhina IuG, Bel'mer SV, editors. Proceeding of the XVIII Congress of Pediatric Gastroenterologists of Russia and the CIS Countries on Actual Problems of Abdominal Pathology in Children. 2011, March 22-24; Moscow, Russian Federation. Moscow: Medpraktika-M; 2011. 5-8 pp. (in Russian).

Shcherbakov AA, Korsunskii AA, Isakov VA. Bolezni organov pishchevareniia u detei pri khelikobakterioze [Digestive apparatus diseases in children with helicobacteriosis]. Moscow: MIA; 2011. 224 p. (in Russian).

Pacifico L, Anania C, Osborn JF, Ferraro F, Chiesa C. Consequences of Helicobacter pylori infection in children. World J Gastroenterol. 2010 Nov 7;16(41):5181-94.

Hagag AA, Amin SM, Emara MH, Abo-Resha SE. Gastric Mucosal Oxidative Stress Markers in Children with Helicobacter Pylori Infection. Infect Disord Drug Targets. 2018;18(1):60-67. doi: 10.2174/1871526517666170502154350.

Szlachcic A, Krzysiek-Maczka G, Pajdo R, et al. The impact of asymmetric dimethylarginine (ADAMA), the endogenous nitric oxide (NO) synthase inhibitor, to the pathogenesis of gastric mucosal damage. Curr Pharm Des. 2013;19(1):90-7. doi: 10.2174/13816128130113.

Wang L, Zhou Y, Peng J, Zhang Z, Jiang DJ, Li YJ. Role of endogenous nitric oxide synthase inhibitor in gastric mucosal injury. Can J Physiol Pharmacol. 2008 Mar;86(3):97-104. doi: 10.1139/y08-003.

Zhang Z, Zou YY, Li FJ, Hu CP. Asymmetric dimethylarginine: a novel biomarker of gastric mucosal injury? World J Gastroenterol. 2011 May 7;17(17):2178-80. doi: 10.3748/wjg.v17.i17.2178.

Münzel T, Sinning C, Post F, Warnholtz A, Schulz E. Pathophysiology, diagnosis and prognostic implications of endothelial dysfunction. Ann Med. 2008;40(3):180-96. doi: 10.1080/07853890701854702.

Coskun S, Kasirga E, Yilmaz O, et al. Is Helicobacter pylori related to endothelial dysfunction during childhood? Pediatr Int. 2008 Apr;50(2):150-3. doi: 10.1111/j.1442-200X.2008.02542.x.

Ohara H, Isomoto H, Wen CY, et al. Expression of mucosal addressin cell adhesion molecule 1 on vascular endothelium of gastric mucosa in patients with nodular gastritis. World J Gastroenterol. 2003 Dec;9(12):2701-5. doi: 10.3748/wjg.v9.i12.2701.

Golikov PP. Rol' oksida azota v klinike neotlozhnykh sostoianii [The role of nitric oxide in an emergency clinic]. Moscow: Medpraktika; 2004. 180 p. (in Russian).

Published

2019-08-01

Issue

Section

Clinical Pediatrics