DOI: https://doi.org/10.22141/2224-0551.13.8.2018.154150

Features of the cytoprotection of gastric and duodenal mucosa in adolescents with chronic gastroduodenitis on the background of food hypersensitivity

V.S. Berezenko, M.S. Krotevich, T.A. Bogdanova, V.V. Zamula, H.E. Kozynkevych

Abstract


Background. The cytoprotective properties of the gastrointestinal tract in children with chronic gastroduodenitis on the background of food hypersensitivity remain understudied. The purpose is to assess the state of the cytoprotective potential of gastric and duodenal mucosa in adolescents with chronic gastroduodenitis on the background of food hypersensitivity by determining the level of trefoil factor (TFF) 1 in gastric mucosa and TFF-2 — in gastric and duodenal mucosa by immunohistochemistry method. Materials and methods. Fifty adolescents (aged 12 to 17 years) were observed and divided into two groups. The first group consisted of 64 % (n = 32) of patients with chronic gastroduodenitis and food hypersensitivity; the second group included 36 % (n = 18) of children with chronic gastroduodenitis without food hypersensitivity. All children underwent morphological and immunohistochemical studies of biopsy specimens with the determination of TFF-1 and TFF-2. Evaluation of TFF-1 and TFF-2 expression was carried out on a point-scale according to the number of stained epithelial cells of the glands of the antrum and goblet cells in the field of view with a 40-fold magnification: 0 points — no color, 1 point — 30 % of stained cells, 2 points — 30–70 % of stained cells, 3 points — 70–100 % of stained cells. Results. A significant expression of TFF-1 (3 points) in the gastric mucosa occurred in adolescents of both groups. According to the results of the study, 62.5 % (n = 20) of children in the first group and 22 % (n = 4) of adolescents in the second group did not have staining of goblet cells (0 points) in duodenal mucosa, (c2 = 7.5; p = 0.008). Weak expression (1 point) occurred in 34.5 % (n = 11) of children in the first group and in the majo­rity of children (67 % (n = 12)) in the second group (c2 = 4.84; p = 0.03). According to the results of statistical analysis, a group of children with food hypersensitivity has 17 times higher risk of atrophic changes in the duodenal mucosa. Conclusions. In children with food hypersensitivity, the cytoprotective properties of the duodenal mucosa are reduced, as evidenced by the absence of TFF-2 expression. In addition, microerosions, a decrease in the number of crypts and Brunner’s glands in the duodenal mucosa are significantly more frequent in the group of children with food hypersensitivity in the absence or weak expression of TFF-2 (p < 0.05).


Keywords


adolescents; chronic gastroduodenitis; trefoil factor; food hypersensitivity; goblet cells

References


Bobrova VI. Zakhvoryuvannya orhaniv hastroduodenalʹnoyi zony u ditey [Diseases of organs of the gastroduodenal zone in children]. Kharkiv: Zoloti Storinki; 2015. 160 р. (in Ukrainian).

Kjellev S. The trefoil factor family - small peptides with multiple functionalities. Cell Mol Life Sci. 2009 Apr;66(8):1350-69. doi: 10.1007/s00018-008-8646-5.

Kim YS, Ho SB. Intestinal goblet cells and mucins in health and disease: recent insights and progress. Curr Gastroenterol Rep. 2010 Oct;12(5):319-30. doi: 10.1007/s11894-010-0131-2.

Hoffmann W. TFF2, a MUC6-binding lectin stabilizing the gastric mucus barrier and more (Review). Int J Oncol. 2015 Sep;47(3):806-16. doi: 10.3892/ijo.2015.3090.

Kouznetsova I, Laubinger W, Kalbacher H, et al. Biosynthesis of Gastrokine-2 in the Human Gastric Mucosa: Restricted Spatial Expression along the Antral Gland Axis and Differential Interaction with TFF1, TFF2 and Mucins. Cell Physiol Biochem. 2007;20(6):899-908. doi: 10.1159/000110450.

Aihara E, Engevik KA, Montrose MH. Trefoil factor peptides and gastrointestinal function. Annu Rev Physiol. 2017 Feb 10;79:357-380. doi: 10.1146/annurev-physiol-021115-105447.

Xiao P, Ling H, Lan G, Liu J, Hu H, Yang R. Trefoil factors: Gastrointestinal-specific proteins associated with gastric cancer. Clin Chim Acta. 2015 Oct 23;450:127-34. doi: 10.1016/j.cca.2015.08.004.

Aamann L, Vestergaard EM, Grønbæk H. Trefoil factors in inflammatory bowel disease. World J Gastroenterol. 2014 Mar 28;20(12):3223-30. doi: 10.3748/wjg.v20.i12.3223.

Du TY, Zhang Y, Zhang Y. Trefoil factor: from laboratory to clinic. Dongwuxue Yanjiu. 2010 Feb;31(1):17-26.

Lebherz-Eichinger D, Tudor B, Ankersmit HJ, et al. Trefoil Factor 1 Excretion Is Increased in Early Stages of Chronic Kidney Disease. PLoS One. 2015 Sep 21;10(9):e0138312. doi: 10.1371/journal.pone.0138312.

Busch M, Dünker N. Trefoil factor family peptides--friends or foes. Biomol Concepts. 2015 Dec;6(5-6):343-59. doi: 10.1515/bmc-2015-0020.

Hensel KO, Boland V, Postberg J, et al. Differential expression of mucosal trefoil factors and mucins in pediatric inflammatory bowel diseases. Sci Rep. 2014 Dec 5;4:7343. doi: 10.1038/srep07343.

Shestopalov A.V., Trofimenko O.V., Shestopalova M.A. Level trefoil peptides (tff-1 and tff-2) in children with chronic gastroduodenitis. Fundamental research. 2012;(10 Pt2):363-366. (in Russian).

Im S, Yoo C, Jung JH, Choi HJ, Yoo J, Kang CS. Reduced expression of TFF1 and increased expression of TFF3 in gastric cancer: correlation with clinicopathological parameters and prognosis. Int J Med Sci. 2013;10(2):133-40. doi: 10.7150/ijms.5500.

Esposito R, Morello S, Vllahu M, Eletto D, Porta A, Tosco A. Gastric TFF1 Expression from Acute to Chronic Helicobacter Infection. Front Cell Infect Microbiol. 2017 Oct 9;7:434. doi: 10.3389/fcimb.2017.00434.




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