DOI: https://doi.org/10.22141/2224-0551.13.5.2018.141560

Level of biological markers of fibrogenesis and angiogenesis in the urine of children with vesicoureteral reflux

N.I. Makieieva, O.O. Morozova

Abstract


Background. Vesicoureteral reflux (VUR) is observed in 1–2 % of children’s population, and its incidence among children with urinary infection achieves 70 %. The objective of this study was to assess the urine levels of transforming growth factor b1 (TGF-b1) and vascular endothelial growth factor (VEGF) as noninvasive markers of VUR and renal parenchymal scarring in children. Materials and methods. Fifty four patients aged 6 months to 16 years with III–V degrees of VUR after endoscopic treatment in a period of clinical and laboratory remission of pyelonephritis were examined. Urine TGF-b1 and VEGF concentrations were measured using commercially kit Platinum ELISA (Austria), according to the manufacturer’s instructions. Results. We have found a significantly higher urine TGF-b1 and VEGF level in children with VUR than in children of control groups (рc–1 = 0.000125 and рc–2 = 0.000081; рc–1 = 0.0000 and рc–2 = 0.000021, respectively). Median urinary TGF-b1 level in patients with VUR and renal scarring was significantly higher than in those with VUR without renal scarring (р1–2 = 0.0269). Median urinary VEGF level in patients with VUR and renal scarring was significantly lower than in individuals with VUR without renal scarring (р1–2 = 0.0163). When studying TGF-b1 and VEGF urine levels, it was found that the Kruskal-Wallis criterion was highly statistically significant, therefore, the statistical characteristics of the corresponding indices differed significantly in the various groups. Conclusions. Thus, levels of urinary excretion of biomarkers of fibrogenesis and angiogenesis can be used as markers for renal parenchymal scarring in children with VUR.


Keywords


vesicoureteral reflux; renal scarring; transforming growth factor β1; vascular endothelial growth factor

References


Arena S, Iacona R, Impellizzeri P, et al. Physiopathology of vesico-ureteral reflux. Ital J Pediatr. 2016 Nov 29;42(1):103. doi: 10.1186/s13052-016-0316-x.

Lakomova DYu, Morozov DA, Morozova OL. Methods for early diagnostics of renal damage in children with vesicoureteral reflux. Bulletin of Medical Internet Conferences. 2013;3(3): 486-489. (in Russian).

Kryganova TA, Dlin VV. The rate of urinary tract abnormalities and the functional state of kidneys in relation to the degree of connective tissue dysplasia in children. Rossiiskii vestnik perinatologii pediatrii. 2016;61(3):81-86. doi: 10.21508/1027-4065-2016-61-3-81-86. (in Russian).

Becknell B, Schober M, Korbel L, Spencer JD. The diagnosis, evaluation and treatment of acute and recurrent pediatric urinary tract infections. Expert Rev Anti Infect Ther. 2015 Jan;13(1):81-90. doi: 10.1586/14787210.2015.986097.

Paintsil E. Update on recent guidelines for the management of urinary tract infections in children: the shifting paradigm. Curr Opin Pediatr. 2013 Feb;25(1):88-94. doi: 10.1097/MOP.0b013e32835c14cc.

Tokarchuk NI, Odarchuk IV, Zayichko NV. Аnalysis of education indicators of fibrosis in pyelonephritis on the background of vesicoureteral reflux in children of early age. Sovremennaya pediatriya. 2015;(70):93-96. doi: 10.15574/SP.2015.70.93. (in Ukrainian).

Borisova TP, Bagdasarova IV. Urinary tract infection in children. Pyelonephritis. Diagnostic approaches and therapeutic tactics in primary health care. Zdorovʹe rebenka. 2012;(39):9-13. (in Russian).

Batyushin MM. Mechanisms of renal scaring from reflux-nephropathy. Vestnik urologii. 2013;(2):43-51. (in Russian).

Krzemień G, Szmigielska A, Turczyn A, Pańczyk-Tomaszewska M. Urine interleukin-6, interleukin-8 and transforming growth factor β1 in infants with urinary tract infection and asymptomatic bacteriuria. Cent Eur J Immunol. 2016;41(3):260-267. doi: 10.5114/ceji.2016.63125.

Kirillov VI, Bogdanova NA, Nikitina SYu. Diagnostic value of determination of urine cytokines in children with urinary tract diseases. Rossiyskii vestnik perinatologii i pediatrii. 2016;61(5):82-88. doi: 10.21508/1027–4065–2016–61–5–82–88. (in Russian).

Simões e Silva AC, Pereira AB, Teixeira MM, Teixeira AL. Chemokines as Potential Markers in Pediatric Renal Diseases. Dis Markers. 2014;2014:278715. doi: 10.1155/2014/278715.

Chichuga E, Nastausheva T, Zvyagina T. Markers of chronic kidney disease in children with obstructive uropathy or vesicoureteral reflux. Farmakologiya. 2015;12(4):407–413.doi:10.15690/pf.v12i4.1421. (in Russian).

Yılmaz S, Özçakar ZB, Kurt Şükür ED, et al. Vesicoureteral reflux and renal scarring risk in children after the first febrile urinary tract infection. Nephron. 2016;132(3):175-80. doi: 10.1159/000443536.

Shamitova EN, Romanov VO. Podocyte markers in urine. Success of modern science and education. 2016;6(11):107-110. (in Russian).

Lee HE, Kim DK, Kang HK, Park K. The diagnosis of febrile urinary tract infection in children may be facilitated by urinary biomarkers. Pediatr Nephrol. 2015 Jan;30(1):123-30. doi: 10.1007/s00467-014-2905-5.

Lakomova DIu. Index of early renal damage in children with vesicoureteral reflux. Saratov Journal of Medical Scientific Research. 2012;8(2):318-324. (in Russian).

Helmke A, von Vietinghoff S. Extracellular vesicles as mediators of vascular inflammation in kidney disease. World J Nephrol. 2016 Mar 6;5(2):125-38. doi: 10.5527/wjn.v5.i2.125.

Arena S, Iacona R, Impellizzeri P, et al. Physiopathology of vesico-ureteral reflux. Ital J Pediatr. 2016 Nov 29;42(1):103. doi: 10.1186/s13052-016-0316-x.

Zaikova NM. Hydroxyprolinuriya’s clinical significance in children with reflux-nephropathy. Nephrologia. 2013;17(3):68-74. (in Russian).

Renata Y, Jassar H, Katz R, Hochberg A, Nir RR, Klein-Kremer A. Urinary concentration of cytokines in children with acute pyelonephritis. Eur J Pediatr. 2013 Jun;172(6):769-74. doi: 10.1007/s00431-012-1914-2.

Merrikhi A, Bahraminia E. Association of urinary transforming growth factor -β1 with the ureteropelvic junction obstruction. Adv Biomed Res. 2014 May 28;3:123. doi: 10.4103/2277-9175.133196.

Morozov DA, Morozova OL, Morrison VV, Lakomova DY. Pathogenic basis and modern prospects in early diagnostics of nephrosclerosis in children with vesicoureteral reflux. Saratov Journal of Medical Scientific Research. 2011;7(1):151-157. (in Russian).

Życzkowski M, Żywiec J, Nowakowski K, Paradysz A, Grzeszczak W, Gumprecht J. Estimation of the relationship between the polymorphisms of selected genes: ACE, AGTR1, TGFβ1 and GNB3 with the occurrence of primary vesicoureteral reflux. Int Urol Nephrol. 2017 Mar;49(3):387-397. doi: 10.1007/s11255-016-1483-9.

Bimpaki E, Bitsori M, Choulaki C, Galanakis E. Vascular endothelial growth factor-A gene polymorphism is associated with congenital renal lesions in children with urinary tract infections. Acta Paediatr. 2017 Aug;106(8):1348-1353. doi: 10.1111/apa.13897.

Hussein A, Askar E, Elsaeid M, Schaefer F. Functional polymorphisms in transforming growth factor-beta-1 (TGFbeta-1) and vascular endothelial growth factor (VEGF) genes modify risk of renal parenchymal scarring following childhood urinary tract infection. Nephrol Dial Transplant. 2010 Mar;25(3):779-85. doi: 10.1093/ndt/gfp532.




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